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Manipulating the mouse embryo : a laboratory manual / Richard Behringer, University of Texas, M.D. Anderson Cancer Center [and three others].

By: Behringer, Richard.
Edition: Fourth edition.Description: xxii, 814 pages : illustrations ; 29 cm.ISBN: 9781936113002 (hardback); 9781936113019 (paper).Subject(s): Mice -- Embryos -- Laboratory manuals | Mice -- Genetic engineering -- Laboratory manuals | Transgenic mice -- Laboratory manuals | Mice as laboratory animals | SCIENCE / Life Sciences / Biology / Developmental Biology | SCIENCE / Life Sciences / Biology / Molecular Biology | MEDICAL / EmbryologyDDC classification: 616.02/7333 Other classification: SCI072000 | SCI049000 | MED025000
Contents:
Genetics and embryology of the mouse: past, present, and future -- Summary of mouse development -- A mouse colony for the production of transgenic and chimeric animals -- Recovery and in vitro culture of preimplantation embryos -- Isolation, culture, and manipulation of postimplantation embryos -- Surgical procedures -- Production of transgenic mice by pronuclear microinjection -- Embryo-derived stem cell lines -- Germ line-competent stem cells derived from adult mice -- Vector designs for pluripotent stem cell-based transgenesis and genome alterations -- Introduction of foreign DNA into embryonic stem cells -- Production of chimeras -- Genotyping -- Parthenogenesis, pronuclear transfer, and mouse cloning -- Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection -- Cryopreservation, rederivation, and transport of mouse strains -- Techniques for visualizing gene products, cells, tissues, and organ systems -- Setting up a micromanipulation lab.
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Item type Current location Call number Status Date due Barcode Item holds
Book Skoltech library
Shelves 		QL737.R6 M2468 2014 (Browse shelf) Available 2000007803
Book Skoltech library
Shelves 		QL737.R6 M2468 2014 (Browse shelf) Available 2000006209
Total holds: 0

Includes bibliographical references and index.

Genetics and embryology of the mouse: past, present, and future -- Summary of mouse development -- A mouse colony for the production of transgenic and chimeric animals -- Recovery and in vitro culture of preimplantation embryos -- Isolation, culture, and manipulation of postimplantation embryos -- Surgical procedures -- Production of transgenic mice by pronuclear microinjection -- Embryo-derived stem cell lines -- Germ line-competent stem cells derived from adult mice -- Vector designs for pluripotent stem cell-based transgenesis and genome alterations -- Introduction of foreign DNA into embryonic stem cells -- Production of chimeras -- Genotyping -- Parthenogenesis, pronuclear transfer, and mouse cloning -- Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection -- Cryopreservation, rederivation, and transport of mouse strains -- Techniques for visualizing gene products, cells, tissues, and organ systems -- Setting up a micromanipulation lab.

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